Yes. One E. coli population evolved the ability to use citrate, which its ancestors could not do, after about 31,500 generations.
Receipt
Redrawn from published values.
Blount ZD, Borland CZ, Lenski RE. Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coli. PNAS. 2008;105(23):7899-906. doi:10.1073/pnas.0803151105





